goat anti mouse gal 9 Search Results


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R&D Systems goat anti mouse gal 9
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Bio-Techne corporation human galectin-9 antibody
Human Galectin 9 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems biotinylated goat anti mouse gal 9 antibody
Biotinylated Goat Anti Mouse Gal 9 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems gal 9 receptor tim 3
Gal 9 Receptor Tim 3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems Hematology goat anti-human gal-9
Goat Anti Human Gal 9, supplied by R&D Systems Hematology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems anti gal 9
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Cell Signaling Technology Inc anti gal 9 antibody
Anti Gal 9 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Techne corporation goat polyclonal antibody
Western blot (WB) analysis of cold immuno-precipitation (IP) products obtained with various anti-gal-9 antibodies. The source of native gal-9 was a protein extract from a murine thymus. Concomitant IP were performed with non-specific mouse IgG1 (negative control), Gal-Nab1 and Gal-Nab2, 9M1 (a commercial antibody directed to human gal-9) and RG9-35 (a commercial antibody directed to murine gal-9). 15 ng of recombinant murine gal-9 M (lane 1) and 25 μg of crude murine thymus extract (lane 2) were used as WB controls. The primary antibody used for WB detection of murine gal-9 was a <t>polyclonal</t> goat serum. The secondary antibody reacted with this primary but also with the immunoglobulin heavy chains eluted from the beads giving strong bands at a molecular weight of about 55 Kd. These bands were of similar intensities for all five IP conditions, suggesting that bead capture of the immune complexes was in the same range of efficiency. The bands resulting from gal-9 staining (36 Kd) were assessed by densitometry (graphs for long and short exposure are presented at the bottom of the figure).
Goat Polyclonal Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Proteintech rabbit anti gal 9 antibody
Western blot (WB) analysis of cold immuno-precipitation (IP) products obtained with various anti-gal-9 antibodies. The source of native gal-9 was a protein extract from a murine thymus. Concomitant IP were performed with non-specific mouse IgG1 (negative control), Gal-Nab1 and Gal-Nab2, 9M1 (a commercial antibody directed to human gal-9) and RG9-35 (a commercial antibody directed to murine gal-9). 15 ng of recombinant murine gal-9 M (lane 1) and 25 μg of crude murine thymus extract (lane 2) were used as WB controls. The primary antibody used for WB detection of murine gal-9 was a <t>polyclonal</t> goat serum. The secondary antibody reacted with this primary but also with the immunoglobulin heavy chains eluted from the beads giving strong bands at a molecular weight of about 55 Kd. These bands were of similar intensities for all five IP conditions, suggesting that bead capture of the immune complexes was in the same range of efficiency. The bands resulting from gal-9 staining (36 Kd) were assessed by densitometry (graphs for long and short exposure are presented at the bottom of the figure).
Rabbit Anti Gal 9 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Proteintech rabbit anti-gal-9 antibody
Western blot (WB) analysis of cold immuno-precipitation (IP) products obtained with various anti-gal-9 antibodies. The source of native gal-9 was a protein extract from a murine thymus. Concomitant IP were performed with non-specific mouse IgG1 (negative control), Gal-Nab1 and Gal-Nab2, 9M1 (a commercial antibody directed to human gal-9) and RG9-35 (a commercial antibody directed to murine gal-9). 15 ng of recombinant murine gal-9 M (lane 1) and 25 μg of crude murine thymus extract (lane 2) were used as WB controls. The primary antibody used for WB detection of murine gal-9 was a <t>polyclonal</t> goat serum. The secondary antibody reacted with this primary but also with the immunoglobulin heavy chains eluted from the beads giving strong bands at a molecular weight of about 55 Kd. These bands were of similar intensities for all five IP conditions, suggesting that bead capture of the immune complexes was in the same range of efficiency. The bands resulting from gal-9 staining (36 Kd) were assessed by densitometry (graphs for long and short exposure are presented at the bottom of the figure).
Rabbit Anti Gal 9 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Jackson Immuno donkey anti goat
Western blot (WB) analysis of cold immuno-precipitation (IP) products obtained with various anti-gal-9 antibodies. The source of native gal-9 was a protein extract from a murine thymus. Concomitant IP were performed with non-specific mouse IgG1 (negative control), Gal-Nab1 and Gal-Nab2, 9M1 (a commercial antibody directed to human gal-9) and RG9-35 (a commercial antibody directed to murine gal-9). 15 ng of recombinant murine gal-9 M (lane 1) and 25 μg of crude murine thymus extract (lane 2) were used as WB controls. The primary antibody used for WB detection of murine gal-9 was a <t>polyclonal</t> goat serum. The secondary antibody reacted with this primary but also with the immunoglobulin heavy chains eluted from the beads giving strong bands at a molecular weight of about 55 Kd. These bands were of similar intensities for all five IP conditions, suggesting that bead capture of the immune complexes was in the same range of efficiency. The bands resulting from gal-9 staining (36 Kd) were assessed by densitometry (graphs for long and short exposure are presented at the bottom of the figure).
Donkey Anti Goat, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
SouthernBiotech goat anti mouse igg fitc
Western blot (WB) analysis of cold immuno-precipitation (IP) products obtained with various anti-gal-9 antibodies. The source of native gal-9 was a protein extract from a murine thymus. Concomitant IP were performed with non-specific mouse IgG1 (negative control), Gal-Nab1 and Gal-Nab2, 9M1 (a commercial antibody directed to human gal-9) and RG9-35 (a commercial antibody directed to murine gal-9). 15 ng of recombinant murine gal-9 M (lane 1) and 25 μg of crude murine thymus extract (lane 2) were used as WB controls. The primary antibody used for WB detection of murine gal-9 was a <t>polyclonal</t> goat serum. The secondary antibody reacted with this primary but also with the immunoglobulin heavy chains eluted from the beads giving strong bands at a molecular weight of about 55 Kd. These bands were of similar intensities for all five IP conditions, suggesting that bead capture of the immune complexes was in the same range of efficiency. The bands resulting from gal-9 staining (36 Kd) were assessed by densitometry (graphs for long and short exposure are presented at the bottom of the figure).
Goat Anti Mouse Igg Fitc, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Western blot (WB) analysis of cold immuno-precipitation (IP) products obtained with various anti-gal-9 antibodies. The source of native gal-9 was a protein extract from a murine thymus. Concomitant IP were performed with non-specific mouse IgG1 (negative control), Gal-Nab1 and Gal-Nab2, 9M1 (a commercial antibody directed to human gal-9) and RG9-35 (a commercial antibody directed to murine gal-9). 15 ng of recombinant murine gal-9 M (lane 1) and 25 μg of crude murine thymus extract (lane 2) were used as WB controls. The primary antibody used for WB detection of murine gal-9 was a polyclonal goat serum. The secondary antibody reacted with this primary but also with the immunoglobulin heavy chains eluted from the beads giving strong bands at a molecular weight of about 55 Kd. These bands were of similar intensities for all five IP conditions, suggesting that bead capture of the immune complexes was in the same range of efficiency. The bands resulting from gal-9 staining (36 Kd) were assessed by densitometry (graphs for long and short exposure are presented at the bottom of the figure).

Journal: PLoS ONE

Article Title: Characterization of neutralizing antibodies reacting with the 213-224 amino-acid segment of human galectin-9

doi: 10.1371/journal.pone.0202512

Figure Lengend Snippet: Western blot (WB) analysis of cold immuno-precipitation (IP) products obtained with various anti-gal-9 antibodies. The source of native gal-9 was a protein extract from a murine thymus. Concomitant IP were performed with non-specific mouse IgG1 (negative control), Gal-Nab1 and Gal-Nab2, 9M1 (a commercial antibody directed to human gal-9) and RG9-35 (a commercial antibody directed to murine gal-9). 15 ng of recombinant murine gal-9 M (lane 1) and 25 μg of crude murine thymus extract (lane 2) were used as WB controls. The primary antibody used for WB detection of murine gal-9 was a polyclonal goat serum. The secondary antibody reacted with this primary but also with the immunoglobulin heavy chains eluted from the beads giving strong bands at a molecular weight of about 55 Kd. These bands were of similar intensities for all five IP conditions, suggesting that bead capture of the immune complexes was in the same range of efficiency. The bands resulting from gal-9 staining (36 Kd) were assessed by densitometry (graphs for long and short exposure are presented at the bottom of the figure).

Article Snippet: A goat polyclonal antibody was used for detection of murine gal-9 by Western blotting (R&D System/Bio-Techne).

Techniques: Western Blot, Immunoprecipitation, Negative Control, Recombinant, Molecular Weight, Staining